The role of pruriceptors in enhancing sensitivity to pruritogens in a murine chronic compression model of dorsal root ganglion

Abstract Chronic pruritus is a symptom that commonly observed in neurological diseases. It has been hypothesized the chronic may result from sensitization of itch-signaling pathways but mechanisms remain obscure. In this study, we established mouse model compression dorsal root ganglion (CCD) and injected various pruritogenic algogenic agents intradermally to calf skin ipsilateral compressed (DRG). Compared naïve mice, significant increase itch-related behaviors was CCD mice after injection pruritogens including histamine BAM8-22, not capsaicin, although all above evoked enhanced pain-related toward site. addition, investigated if pruritogen-evoked activities DRG neurons were model. vivo calcium imaging revealed exhibited responses BAM8-22. Immunoflorescent staining also showed receptor H1 capsaicin TRPV1 significantly upregulated neurons. Our findings indicated primary pruriceptive underlie itch sensation play role